Dr Booth completed a BSc (Health and Exercise Science) and MSc (Biomedical Science) in Cardiff in 2007, before the Award of a Wellcome Trust PhD Studentship at the University of Liverpool. Here he received an MRes (Molecular and Cellular Physiology), prior to PhD studies in the lab of Professor Steve Royle, investigating the role of clathrin at the mitotic spindle. Pursuing his interest in cell division Dr Booth joined the lab of Professor Bill Earnshaw in 2012, at the Wellcome Trust Centre for Cell Biology, Edinburgh. As a post-doctoral researcher Dr Booth established and applied numerous advanced imaging and proteomics techniques to investigate fundamental properties of chromosome structure. To add more translational impact to his work Dr Booth undertook further post-doctoral training with Professor Dies Meijer at the Centre for Discovery Brain Sciences, in 2016. Here he generated and exploited transgenic animal models to dissect the molecular pathways linked to a variety of disease states.
In 2019 Dr Booth was awarded a Nottingham Research Fellowship, allowing him to establish his own lab at the brand new Biodiscovery Institute, an endeavour that houses ~350, academics, clinicians, researchers and PhD students across five floors of state-of-the-art laboratories and research space
We are a multidisciplinary lab with numerous core strengths and have a strong history of developing advanced imaging pipelines, such as correlative light electron microscopy (CLEM). Enhanced versions of this includes 3D-CLEM and Super-CLEM.
In addition we are experienced in a range of proteomics techniques, in particular proximity based approaches used to map protein neighbourhoods.
We have successfully applied these systems to a variety of disciplines, ranging from spindle microtubules and chromosome architecture to nuclear membranes and myelinating axons.
REMNANT, L., BOOTH, D. G., VARGIU, G., SPANOS, C., KERR, A. R. W. and EARNSHAW, W. C., 2019. In vitro BioID: mapping the CENP-A microenvironment with high temporal and spatial resolution: Mol Biol Cell Mol Biol Cell. 30(11), 1314-1325
SAMEJIMA, K., BOOTH, D. G., OGAWA, H., PAULSON, J. R., XIE, L., WATSON, C. A., PLATANI, M., KANEMAKI, M. T. and EARNSHAW, W. C., 2018. Functional analysis after rapid degradation of condensins and 3D-EM reveals chromatin volume is uncoupled from chromosome architecture in mitosis: J Cell Sci J Cell Sci. 131(4),